Study on Action Mode of Sphingosine 1-Phosphate in Rat Hepatocytes

Study on Action Mode of Sphingosine 1-Phosphate in Rat Hepatocytes

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Sphingosine-1-phosphate (S1P) acts on a read more set of G protein-coupled receptors in the plasma membrane and also as a second messenger in certain cell types.There are two possible pathways to mobilize intracellular Ca2+ concentration by S1P.One is through phospholipase C, and the other is through intracellular Ca2+ channels operated by S1P.

The Mn2+ quenching method was applied to elucidate the action mode of S1P-induced Ca2+ mobilization in rat catherine lansfield ombre rainbow clouds eyelet curtains hepatocytes.In permeabilized hepatocytes, inositol trisphosphate induced Mn2+ quenching, and it was blocked by heparin.However, S1P did not induce Mn2+ quenching.

Results suggest that S1P did not mobilize Ca2+ through intracellular Ca2+ channels.Keywords:: hepatocyte, sphingosine 1-phosphate, second messenger.